Composite

Part:BBa_K4719018:Design

Designed by: Auguste Stankeviciute   Group: iGEM23_Vilnius-Lithuania   (2023-09-06)


pKARA_RT3 styrene monooxigenase for indigo synthesis in K. xylinus


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1226
    Illegal SpeI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1226
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1226
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1226
    Illegal SpeI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1226
    Illegal SpeI site found at 37
    Illegal NgoMIV site found at 92
    Illegal NgoMIV site found at 134
    Illegal NgoMIV site found at 503
    Illegal AgeI site found at 689
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

.


Source

synthetic

References